A mad scientist discovered a new gene (NG) in the green anoles that Dr. Jackson

Question

A mad scientist discovered a new gene (NG) in the green anoles that Dr. Jackson does research on. Dr. Jackson wanted to study this gene in her experiments designed to look at tail regeneration (regrowing of the tail after tail ejection). The mad scientist told Dr. Jackson the sequence of the gene including where the exons and introns occurred in the sequence. The mad scientist also gave Dr. Jackson the probe that he used to perform a Northern Blot looking at tissue expression of the gene in the heart and stated that there was no nonspecific binding of the probe. In his experiment, the probe gave a product of 1,000 bp. Dr. Jackson isolated tips of the tail from lizards after tails had been ejected at time points 0, 1 week, and 2 weeks and performed a Northern Blot looking at this gene. Lane 1- NG 0 time point; Lane 2-NG 1 week, Lane 3: NG 2 weeks; Lane 4= -actin 0 time point; Lane 5--actin 1 week, Lane 6--actin 2 weeks A: (2 pts) For this experiment, would I want to isolate DNA, RNA, or protein? B: (4 pts) Describe the results seen on the Northern Blot in Lanes 1-3 without looking at Lanes 4-6 and give one possibility of why I saw something other than the mad scientist. (What does this suggest about this gene?) C: (4 pts) Lanes 4-6 are a housekeeping gene. When looking at these results, how does it affect the results seen in Lanes 1-3? 1 23 45 6 1000bp 800 bp 600 bp

Explanation / Answer

(A) you have to isolate protein because you have to see the gene involved in the tail formation, this gene will form protein after transcription,

(B) the lane 1 this is the new gene at time point zero and the gene is giving band of 1000 bp, this is initial position.

Lane 2 & 3 the size of the gene is reduced and the expression of gene is different in lane 2 then lane 1,

there is no change in lane 2 and 3 , means there is no change of expression of gene in week 1 and week 2,

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