Match the correct technique to the goal. GFP translational fusion testing transc

Question

Match the correct technique to the goal.

GFP translational fusion

testing transcription factor binding to a promoter

test if two protein interact

study a purified protein in vitro

determine exact sequence to which a transcription factor binds on DNA

identification of unknown genes necessary for a particular phenotype

examine the expression of a particular gene in living cells

localize proteins within living tissue

yeast two-hybrid

GFP transcriptional fusion

CRISPR

transposon mutagenisis and phenotype selection

DNA footprinting

EMSA

affinity chromatography

  

GFP translational fusion

testing transcription factor binding to a promoter

test if two protein interact

study a purified protein in vitro

  

determine exact sequence to which a transcription factor binds on DNA

identification of unknown genes necessary for a particular phenotype

examine the expression of a particular gene in living cells

A.

localize proteins within living tissue

B.

yeast two-hybrid

C.

GFP transcriptional fusion

D.

CRISPR

E.

transposon mutagenisis and phenotype selection

F.

DNA footprinting

G.

EMSA

H.

affinity chromatography

Explanation / Answer

GFP translational fusion - localize proteins within living tissue

testing transcription factor binding to a promoter -EMSA

test if two protein interact -yeast two-hybrid

study a purified protein in vitro - affinity chromatography

determine exact sequence to which a transcription factor binds on DNA -DNA footprinting

identification of unknown genes necessary for a particular phenotype -transposon mutagenesis and phenotype selection

examine the expression of a particular gene in living cells -GFP transcriptional fusion

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