1. I am studying the interaction of proteins PON3 and PON2 in cancer. The names
ID: 212390 • Letter: 1
Question
1. I am studying the interaction of proteins PON3 and PON2 in cancer. The names listed above each wel below are different cancer cell lines (you need to know ABSOLUTELY NOTHING about these names!) If am studying both PON3 and PON2, which cancer cell line(s) would be good to use? If I want a control cell line that expresses NEITHER PON3 or PON2, which line(s) would be good to use? PON3 PON2 2. In the Inositol Phospholipid Pathway, DAG recruits PKC (which acts just like PKA except on different substrates) Here are two western blots showing the different variations of PKC (labeled below). Each western blot is probed with 2 antibodies-one that picks up all PKC variants, the other that only recognizes PKC phosphorylated at Thr638. Which of these variants is NOT phosphorylated:? The Epsilon (e) sample has a significantly thinner/lighter phosphorylation band than on the total PKC blot (most of the other variations are pretty close). What does this likely tell you about how much of E sample is phosphorylated at Thr638? Lastly, could the kinase that phosphorylated this Threonine likely phosphorylate any other amino acid?Explanation / Answer
A549 will be best as it has expression of both Pon2 and Pon3.
Delta as it has no band in 1st lane for phosphorus pic
B activator it will be dinded
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