1. I am studying the interaction of proteins PON3 and PON2 in cancer. The names
ID: 212429 • Letter: 1
Question
1. I am studying the interaction of proteins PON3 and PON2 in cancer. The names listed above each wel below are different cancer cell lines (you need to know ABSOLUTELY NOTHING about these names!) If am studying both PON3 and PON2, which cancer cell line(s) would be good to use? If I want a control cell line that expresses NEITHER PON3 or PON2, which line(s) would be good to use? PON3 PON2 2. In the Inositol Phospholipid Pathway, DAG recruits PKC (which acts just like PKA except on different substrates) Here are two western blots showing the different variations of PKC (labeled below). Each western blot is probed with 2 antibodies-one that picks up all PKC variants, the other that only recognizes PKC phosphorylated at Thr638. Which of these variants is NOT phosphorylated:? The Epsilon (e) sample has a significantly thinner/lighter phosphorylation band than on the total PKC blot (most of the other variations are pretty close). What does this likely tell you about how much of E sample is phosphorylated at Thr638? Lastly, could the kinase that phosphorylated this Threonine likely phosphorylate any other amino acid?Explanation / Answer
1.
A thrilling query is how tumors gain an growth in PON2 or PON3 expression, and this must be a prime goal of future studies. Certainly, there may be no well-known answer to this question.
A point of interest is why some tumors upregulate PON2 or PON3. One of the hallmarks of cancer is resistance to cell death. It has been discovered that paraoxonases 2 and three provide a safety in opposition to mitochondrial cellular dying. Their overexpression diminished susceptibility to unique chemotherapeutics in cell culture.
So, both PON3 & PON2 lines are necessary when studying a cancer
2.
The antibodies that pick all the PKC variants is not phosphorylated. The purified anti-PKC Phospho (Thr638) antibody detects PKC only when phosphorylated at threonine 638.
3.
yes, the kinase that phosphorylated Threonine can phosphorylate other amino acids too like serine, Tyrosine etc.
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