1. The Following questions pertain to the following experiment. a) The enzyme en

Question

1. The Following questions pertain to the following experiment.

a) The enzyme enolase catalyzes the dehydration of 2-phosphoglyceric acid. Sodium fluoride exerts its inhibitory effect by tying up the metal ions necessary for the activity of the enzyme. What metal ion is affected?

b) why is the lactic acid enzyme called dehydrogenase rather than oxidase?

c)) What is the effects of sodium fluoride on the fermentation process?

Fermentation 1. Label three clean small test tubes A, B, and C. Prepare them as follows (yeast suspensions settle; shake before use). Tube A: Fill tube one-third full with the 2.5% yeast suspension. Add water to Tube B: Fill tube one-third full with the 2.5% yeast suspension. Add 0.5 M Tube C: Fill tube one-third full with the 2.5% yeast suspension. Add 0.5 M fill tube completely (enough to see the surface tension). glucose solution to fill tube completely. fluoride solution to fil1 tube completely glucose solution to fill tube two-thirds full Add 0.1 M. sodium

Explanation / Answer

a) The enzyme enolase catalyzes the dehydration of 2-phosphoglyceric acid. Sodium fluoride exerts its inhibitory effect by tying up the metal ions necessary for the activity of the enzyme. What metal ion affected? Mg 2+

b) why is the lactic acid enzyme called dehydrogenase rather than oxidase? oxidase will oxidize oxygen and dehydrogenase catalyzes both reactions that is the main reason behind calling it as dehydrogenase.

c)  The enzyme enolase catalyzes the conversion of phosphoglycerate into phosphoenolpyruvate. This reaction depends on Magnesium ions and the reaction is inhibited by fluoride ions (sodium fluoride). sodium fluoride precipitates Magnesium ions and therefore inhibits the pathway. so, pyruvate cannot be formed and fermentation of pyruvate to ethanol and CO2 will be inhibited.

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