One ml of the unknowns carbohydrate is combined with 5.0 ml of Benedict\'s reage

Question

One ml of the unknowns carbohydrate is combined with 5.0 ml of Benedict's reagent in large tes inisat he tube are mixed and heated in a boiling water bath for 5 minutes. Greenish red precipitation indicates a positive test T ransfer 5 drops of the unknown carbohydrate in small test tubes and add two drops of lodine-I solution. The immediate formation of a vivid blue color indicates amylose or starch. 12.1.7 Data analysis of Unknown Set # Resorcino Benedicts Molisch Test Test Test Test Solution A Solution B | - | ellow Solution C Solution D Solution E RED Solution F reen Such) oran cear Ptir K Indicate the sugar in six unknown solution and justify your answer SolutionA: Solutlon B: Solutlon C: Solution D: Solutlon E: Solution F:

Explanation / Answer

Solution A contains a reducing sugar having ketone as the functional group that gets reduce to give red color. The reducing sugar in this case can be either a monosaccharide or a disaccharide.

Solution B contains starch solution as it is able to give Blue black color as ar result of addition of triiodide.

Solution C contains a reducing disaccharide sugar with aldehyde as the functional group present because it is able to give both Benedict and Molisch test. Solution D contains no carbohydrate molecules as it does not give any positive results for any of these tests.

Solution E contains a non reducing monosaccharide sugar which has ketone group present as the functional group because it is able to give only Molisch test.

Solution F contains a reducing disaccharide sugar which has aldehyde as the functional group present in it because it can give both Molisch and Benedict test.

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