D. What is the size of the uncut plasmid? -is it 8bp or 20-8bp? Also can you ple
ID: 213207 • Letter: D
Question
D. What is the size of the uncut plasmid? -is it 8bp or 20-8bp?Also can you please check my numbers on the last problem regarding fragment size? Numbers are 1644bp, 653bp, and 2064bp. DNA LAB HOMEWORK Below is a pGEN 101 plasmid with restriction sites for BamHl and EcoRI. Several restriction digests were done using these two enzymes either alone or in combination. Kb stands for kilobases, or thousands of base pairs. Plasmid Gel lanes 6 Kb Bam Hl Bam HI 20 Kb 11 Kb Bam HI 8 Kb 4 Kb Eco RI 3 Kb a. Which lane shows a digest with BamHI only? b. Which lane shows a digest with EcoRI only? I c. Which lane shows d, what is the size of the uncut plasmid?- Below is another plasmid, pBR322. If you digest this plasmid with Sall, EcoRI and Ndel, how many fragments the fragments when the plasmid was cut with both EcoRI and BamH V would you get and what size would they be? Digsien of B322 pad nh HindIII BamHI Pst 200b tet amp pBR322 4361 bp
Explanation / Answer
D) What is the size of the uncut plasmid?
20 Kb
The plasmid is uncut, therefore, it will be the size of whole plasmid that is 20 Kb.
Digestion of pBR322 with EcoRI, SalI, and Ndel will produce 3 fragments. The sizes of the fragments are 1644bp (2295 - 651), 653bp ((4359 - 651) +2), and 2064bp (4359 - 2295).
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