25. Current Total cells/cm Fold Expansion Previous Total cels/om 3.71e8(aam 1.27

Question

25. Current Total cells/cm Fold Expansion Previous Total cels/om 3.71e8(aam 1.2706(olam Doubling Time Ln(Fold Expansion) 207 (days) Doubling Time -Ln(2) Time remaining in culture 2 days (days) ls Required for 8.00e (alm)Expansion 26. What does It mean to be aseptic? 27. Microorganisms and pathogens such as bacteria, viruses, and archaea are the only concern in a clean room environment. TRUE / FALSE 28. Isopropyl Alcohol (IPA) kills all microorganisms and other potential contaminants. TRUE / FALSE 29. Dead bacteria are still a potentially lethal contaminant. TRUE / FALSE 30. How might you detect that a cell culture is contaminated? Dilutions 31. Why would you need to dilute something?

Explanation / Answer

26) Aseptic means using practices and procedures to prevent contamination from pathogens. It involves applying the strict rules to minimize the risk of infection.

27) Microorganisms and pathogens such as bacteria, virus, archaea are the only concern in a clean room environment. False

28) Isopropyl alcohol kills all microorganisms and other potential contaminants. False

29) Dead bacteria still a potentially lethal contaminant. False

30) If the cell culture is contaminated then the culture color will be changed drastically. In addition, if we prepare plate for this culture, we will able to see colonies with different morphologies.

31) We dilute something to reduce it’s concentration. In microbiology, dilution gives us the isolated colony which is very useful. It will be easier to pick a single colony for future procedure.

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