Arrange the following components of replication in the order in which they first

Question

Arrange the following components of replication in the order in which they first act: ligase, helicase, primase, single-stranded binding protein, initiator protein. (1pt)

Which key replication enzyme(s) are missing from the protein components listed in Q7 and Q8a)? What do they do? (2pts)

Why might a eukaryotic cell have more “replisome” components than a prokaryotic cell? (2pts)

Why do eukaryotic chromosomes have multiple origins of replication, whereas prokaryotic chromosomes typically have only one origin? (2pts)

Explanation / Answer

Arrange the following components of replication in the order in which they first act: ligase, helicase, primase, single-stranded binding protein, initiator protein.

answer: initiator protein, helicase, single-strand binding protein, primase, ligase

Which key replication enzyme(s) are missing from the protein components listed in Q7 and Q8a)?

If the above question is Q7 and Q8a we can say an important enzyme that is missing in the above ques is DNA pol I and gyrase.

What do they do?

DNA pol I is impotant enzyme participating in DNA repair and replication while gyrase relaxes the supercoiling caused due to unwinding.

Why might a eukaryotic cell have more “replisome” components than a prokaryotic cell.

There are around13 components of the E.coli replisome and at least 27 in the replisomes of yeast and mammals (eukaryotes). This might be due to higher complexity of the eukaryotic template. Eukaryotic cell is more complex than prokaryotic cell. The eukaryotic replisome in addition to performing all functions of the prokaryotic replisome disassemble and reassemble the protein-DNA complexes called nucleosomes

Why do eukaryotic chromosomes have multiple origins of replication, whereas prokaryotic chromosomes typically have only one origin?

Eukaryotic chromosomes have multiple origins while prokaryotic chromosomes have one origin of replication because eukaryotic chromosomes are much larger, so multiple origins are needed to replicate the entire chromosome in a short amount of time. However prokaryotic chromosomes are small, so having only one origin will be sufficient

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