loioll Can you draw about the t Ur this novel nucleic acid? Phosphorus is requir

Question

loioll Can you draw about the t Ur this novel nucleic acid? Phosphorus is required to synthesize the deoxyribonucleoside triphosphates used in DNA replication. A geneticist grows some E. coli in a medium containing nonradioactive phosphorus for many generations. A sample of the bacteria is then transferred to a medium that contains a radioactive 32 are removed immediately after the transfer and after one and two rounds of replication. Assume that newly synthesized DNA contains 3?P and the original DNA contains nonradioactive phosphorus. What will be the distribution of radioactivity in the DNA of the bacteria in each sample? Will radioactivity be detected in neither, one, or both strands of the DNA? *22. A line of mouse cells is grown for many generations in a

Explanation / Answer

In the beginning, the sample was removed directly after transfer, no incorporation of 32P should be there into the DNA because replication in the medium containing 32P has not still happened. Following one cycle of replication in the 32P-containing medium, one strand of recently synthesized DNA molecule will include 32P, while another strand will include only nonradioactive phosphorous. After two rounds of replication in the 32P-containing medium, 50% of the DNA molecules will have 32P in both strands, while the rest 50% will contain 32P in one strand and nonradioactive phosphorous in the other strand.

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