Investigators frequently use colorimetric tests to check for the presence of cer

Question

Investigators frequently use colorimetric tests to check for the presence of certain chemicals. For example, when the vitamin riboflavin is present with certain reagents it turns a solution bright yellow. When the vitamin pyridoxine is present with other reagents it turns pink. Suppose you are given three test tubes, one containing riboflavin, the other pyridoxine and a third containing an unknown (riboflavin, pryidoxine, or both). You are also given the necessary reagents.
(a) How would you check your unknown?





(b) What results would you expect if your unknown contained riboflavin, pryidoxine, or both?

Explanation / Answer

a. For checking the unknown, we would use the reagents and then detect riboflavin by checking for bright yellow and for pyridoxine by checking for pink color. Now check for the third tube for the color that it develops which would give us a hint of the third solution. b. If both the compounds riboflavin and pyridoxine are in same concentrations, then one would expect a mixture of solution to have a combination color of yellow and pink that gives slight orangisth tinge.

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