55. You treat cells with Compound Y and perform qPCR to determine the impact of

Question

55. You treat cells with Compound Y and perform qPCR to determine the impact of this treatment on the level of mRNA for GeneX. You produce the following figure.

O 5 10 15 20 25 30 35 40

Cycle

From the figure above, you conclude that Compound Y:

a) Reduces transcription of GeneXby 3 fold

b) Reduces transcription of GeneXby 6 fold

c) Reduces transcription of GeneXby 8 fold

d) Increases transcription of GeneXby 6 fold

e) Increases transcription of GeneX by 8 fold

56. 'l'he fluorescence reading in the figure from the previous question is being generated by:

a) The binding of SYBR Green to mRNA

b) The binding of SYBR Green to single-stranded DNA

c) The binding of SYBR Green to double-stranded DNA

d) The binding of SYBR Green to double-stranded RNA

e) The binding of SYPRO Ruby to mRNA

57. Which of the following statements on the TATA binding protein (TBP) is not true:

a) TBP recognizes an AT-rich sequence, known as the TATA box.

b) TBP is used by all three classes of eukaryotic RNA polymerases.

c) TBP bends DNA by opening up the major groove.

d) TBP is used for transcription initiation of all genes, even when a TATA box is not present in the core promoter.

e). TBP is recruited to near position -30 of the promoter through proteins known as TBPassociated factors (TAFs).

58. Which of the following is true of the electrophoretic mobility shift assay but not the DNA footprinting assay:

a) the technique can be used to identify binding of a protein to a specific segment of DNA

b) the technique involves the resolution of samples by nondenaturing gel electrophoresis

c) the technique involves the amplification of radiolabeled DNA by PCR

d) the technique can identify the precise location of protein binding to a sequence of DNA

e) the technique involves the cleavage of protein-bound DNA using DNase I or free radicals

59. Differential RNA processing may result in:

a) a shift in the ratio of mRNA produced from two adjacent genes.

b) inversion of certain exons in the mature mRNA.

c) the crossover of exons between two different pre-mRNA transcripts

d) the production of the same protein from two different genes.

e) the production of two proteins with different activities from a single gene.

Explanation / Answer

Answer

55. option e) Increases transcription of GeneX by 8 fold

56. option b) The binding of SYBR Green to single-stranded DNA

57. option e). TBP is recruited to near position -30 of the promoter through proteins known as TBPassociated factors (TAFs).

58. option b) the technique involves the resolution of samples by nondenaturing gel electrophoresis

59. option e) the production of two proteins with different activities from a single gene

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