19. Use the qPCR diagram below to answer the following questions: a. (1 points)

Question

19. Use the qPCR diagram below to answer the following questions: a. (1 points) In which sample is the gene of interest more highly expressed (place an "X next to your choice)? Sample A Sample B Gene of interest Sample A Gene of interest Sample B Control Gene Sample B Control Gene Sample A b. (1 points) Did the control gene e work as expected (place an X next to your choice)? 12 15 18 Yes Cycle Threshold No (0.5 points) What is the fold difference in expression between the gene of interest in sample A versus sample B? Show your calculations c. Fold Difference

Explanation / Answer

1. gene of interest in sample A

as it is appearing at cycle 15 , whereas sample B at cycle 18.

2.b. Yes . the control gene is behaving as expected.

as both the control gene gene are expressed almost equally.

3.

delta Ct , sample A= (ct target-ct ref) = (15-12)=3

delta ct, sample B=( ct target-ct ref)= (18-12)=6

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