4. You are a research assistant in a lab that studies nucleic acids. Your adviso
ID: 214397 • Letter: 4
Question
4. You are a research assistant in a lab that studies nucleic acids. Your advisor gave you four tubes for analysis. Each of these tubes differs in its contents by the source of its nucleic acids: mouse cytoplasm (single-stranded RNA), yeast nuclei (double-stranded DNA), rotavirus (double-stranded RNA), and parvovirus (single-stranded DNA). The approximate nucleotide base composition of each sample is given in the table below.
Tube
A
C
U
T
G
1
32
17
0
32
19
2
34
16
15
0
35
3
30
21
0
26
23
4
33
16
34
0
17
Which tube contains the rotavirus (double stranded DNA)? Explain how you know it’s this tube. (2pts)
5. Imagine it is the early 1900s and the nature of genetic material is not yet known. You believe that protein is the most likely candidate for holding information, rather than DNA (which you regard to be some form of “nuclear excrement”).
Make an argument supporting this position (purely from a theoretical standpoint, of course!). (2pts)
Explain how either [Avery, MacLeod, and McCarty] or [Hershey and Chase] contributed to our understanding of DNA as the genetic material. (1.5pts)
How did Rosalind Franklin contribute to Watson and Crick’s elucidation of the structure of DNA? (1.5pts)
What are Chargaff’s rules, and how did Chargaff’s Rules contribute to Watson and Crick’s elucidation of the structure of DNA? (2pts)
6. In the diagram below, the arrow indicates the direction of movement of a replication fork.
Which strand, top or bottom, is the leading strand? Explain. (1pt)
On which strand, top or bottom, would you expect to find Okazaki fragments? Explain. (1pt)
Where would you find the “replisome”? (1pt)
How many primase molecules are needed on the leading strand? The lagging strand? (1pt)
Tube
A
C
U
T
G
1
32
17
0
32
19
2
34
16
15
0
35
3
30
21
0
26
23
4
33
16
34
0
17
Explanation / Answer
The tube 4 contain material of our interest . We can explain this in the basis of that DsRNA contain more amount of uracil than other.
Question number 5- genetic material must be present in all the cells and protein present in all the cells. More ever proteins show complexity as the cells show complex nature.
Now these scientists show that proteins can't replicate themselves as DNA . Further studies show that proteins are coded by DNA. SO DNA is the base of genetic material.
Franklin proposed X-RAY pattern which show the base of DNA IN which they stick together .more ever this diagram also show the minor and major groove of DNA .
CHARGOFF RULES - A+T+C+G = 1.
A+G/ T+G =1
QUESTION NUMBER 6- The strand in which 3'OH is formed is the leading strand . And another is the legging strand.
The primase required is according to the replication unit.
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