Analyze the Data 8-2: DNase Digestion and Gene Expression 2 . Nuclei have been i
ID: 214905 • Letter: A
Question
Analyze the Data 8-2: DNase Digestion and Gene Expression
2. Nuclei have been isolated from a stem cell line at 0, 1, 2, and 4 days after the cell line has been induced to differentiate into muscle type cells. The nuclei are subjected to digestion with DNase, the DNA is then extracted from the nuclei and cleaved with a restriction enzyme that cleaves the DNA external to genes X and Y. The digestion products are run on a gel and analyzed by Southern blots using a labeled probe that binds to either X or Y DNA (or RNA). The results of this experiment are shown on the Southern blot below at the left. The experiment is repeated, but without DNase, and the results are shown on the Southern blot below in the middle. Finally, RNA, extracted from the cells at days 0, 1, 2 and 4 after induction, is run on gels which are then blotted and probed for the presence of X or Y RNA, as shown in the Northern blot below, right.
a. Why do the patterns of bands differ between the two Southern blots?
Days of induction01 2 4 0 1 24 Probe X Probe Y | Southern blot Southern blot Northern blotExplanation / Answer
in first southern blot, we treated the sample with DNase protein which cleaves the DNA . in question for figure 1 it is stated that probe binds with DNA or RNA. so at 0 day DNA of X is more and gradually decreases. at 4th day the X gene is not active so it is not visible in 4th day southern blot while gene Y is not active at 0 day but active at 4th day.
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