estriction nucleases EcoRI ou have a circular piece o HindlI, and Notl at the po

Question

estriction nucleases EcoRI ou have a circular piece o HindlI, and Notl at the positions indicated on the "plasmid map" below. Considering this plasmid map, which of the following statements would be untrue? Notl EcoRI EcoRI Hindlll Figure Q10-6 a. Cleavage of the plasmid with Notl will produce a single linear DNA product. b. Cleavage of the plasmid with Notl and HindIll will produce multiple DNA c. Cleavage of the plasmid with EcoRI and Notl will produce multiple DNA d. Cleavage of the plasmid with HindIII and EcoRI will produce linear DNA e. None of the above segments, one of which will not be a substrate for EcoRI. segments, one of which is not a substrate for HindllI. fragments of unequal size.

Explanation / Answer

Answer1 =c

Cleavage of plasmid by Ecor1 and Not1 will produce3 segments( multiple dna) ,, one of segment is from Not1 to Ecor1 contains hind 111 site also..

Therefore it can cut there and make 2 segment of this one segment.

Answer2= d

FRAP( Florescence recovery after photo bleaching)  

In this bilayer is uniformly labelled with fluroscent tag and this label is selectively photobleched by small fast light.

Intensity in bleached area is monitored as dye diffuses out and new dye diffuses in and finally a new uniform colour appears.

**if procedure used to evaluate a membrane protein then we used the GFP( green fluorescent protein)

Answer3= mechanisms through which cells are able to restrict lateral movement of proteins are:

1)physical.obstacles by immobolile molecules that are sufficiently crowded can obstruct the free passage

2)electrosatic attraction and repulsion = a highly charged molecule is repled by molecules carrying same charge on th cell.membrane and attracted by other molecule.which carries opposite charge.

3)preferrentialltly retain and exclusion of certain molecules by membrane

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