t can be desirable to produce eukaryotic proteins in prokaryotes such as E. coli

Question

t can be desirable to produce eukaryotic proteins in prokaryotes such as E. coli. To do this several DNA sequences must be joined or cloned together. Place the labels in the appropriate position to allow for the expression ofthe insulin protein in a prokaryoic cell cDNA is DNA that is synthesized from the mature mRNA from eukaryotic cells using the enzyme reverse transcriptase. 5' end 3' end Poly A signal sequence Genomic clone of insulin Rho terminator 95 CAT box -10 TATA -25 TATA box cDNA of insulin 35 Sequence

Explanation / Answer

-35 Sequence

-10 TATA

cDNA of insulin

Rho terminator

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PolyA tail in prokaryotes is meant for degradation.

-25 TATA box, -95 CAT box are found in eukaryotes.

Genomic clone consist of introns which cant be modified by prokaryotes.

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