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This method can be used tosterilize a liquid media without harming heat labilein

ID: 2159 • Letter: T

Question

This method can be used tosterilize a liquid media without harming heat labileingredients.

Autoclaving

Pasteurization

Freezing

Cold filtration

Addition of ethylene oxide to themedia

Consider two parallel cultures.They are in all ways identical except that one contains testsubstance X. You observe that a bacterium you are studying growsequally well in both media. Therefore, test substance X is___________.

A macronutrient

A micronutrient

An essential nutrient

A toxin

Not a nutrient

A microbe uses CO2 asits sole source of carbon and oxidizes H2 as its sourceof energy, to which nutritional group does this microbe belong?

Photoautotroph

Chemoorganotroph

Photoorganotroph

Chemoautotroph

A mutant bacterium does not producea functional hydroxamate. This bacterium would have difficultyobtaining ___________.

Phosphate

Iron

Ammonia

Thiosulfate

Hydrogen sulfide

A medium containing methylene blueand incubated at 55º C enriches for ______.

Mesophilic mycoplasma

Hyperthermophilic gramnegatives

Gram positives

Thermophilic gram negatives

Thermophilic gram positives

Bacterium “A” has agrowth rate constant of 4 doubles/hour. Bacterium “B”has a generation time of 0.25 hours/double. Which bacterium growsfaster?

“A” grows faster than“B”

“B” grows faster than“A”

A” and “B” growat the same rate

No conclusion can be made from thisdata

Assume you inoculated 100 cellsinto 100 ml of nutrient broth. You then inoculated 100 cells of thesame species into 200 ml of nutrient broth. After incubation for 12hours, you should have

More cells in the 100 ml

More cells in the 200 ml

The same number of cells inboth.

_________ halodurics useK+ as their osmolyte.

All

Some

No

A medium containing 3.2% NaCl and0.025% phenethyl alcohol would enrich for _________.

Nonhalophiles

Gram-negative halodurics

Gram-negative halophiles

Gram-positive hyperhalophiles

Gram-positive halophiles

The value of the minimum inhibitoryconcentration depends upon_________.

The microbe’s intrinsicsensitivity to the antimicrobial

The rate at which the antimicrobialdiffuses through the agar

Both A and B

Neither A nor B

Which enzyme would be used toneutralize the activity of the [OH·] free radical?

Superoxide dismutase

Catalase

Peroxidase

Nitrogenase

None of the above

Which of the following cansterilize?

70% ethanol

10% sodium hypochorite solution

Ethylene oxide

Mecurochrome

All of the above

You are developing a new line ofprotease containing laundry detergent. You plan on marketing thedetergent to the NFL to remove grass and blood stains from theiruniforms. You most likely isolated the protease from which group ofbacteria?

Acidophile

Neutrophile

Alkalophile

Strict anaerobes

Non-halophile

True or False: Fungi are betterosmophiles than most bacteria.

True

False

The cell density of population ofmicrobes expands six-thousand fold. Approximately how many timesdid the population double?

9

10

12

14

16

Thirty-six colonies grew onnutrient agar plate from 500l of undiluted sample in a standardplate count. How many cells / ml were in the original sample?

17,000

900

18

36

72

Explanation / Answer

1. pasteurization 2. not a nutrient post the rest again...too many questions in 1 post, againstCramster policy
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