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8:49 If the pBR322 plasmid (below) were completely digested with the two restric

ID: 216499 • Letter: 8

Question

8:49 If the pBR322 plasmid (below) were completely digested with the two restriction enzymes BamHI and HgiEII, the resulting fragments would be this large: 2 pts 2 pts 2 pts 2 pts Recognition stepositions are given in parentheses) Eco (4359 EcoV (187 a(4033) nd (29) Fsl (262) 2 pts Sall (651) 2 pts Putl (361 Aod (652 2 pts 2 pts 2 pts 2 pts 2 pts pBR322 4361bp Dral (3251 3232) Fopl (1456 12787 2 pts (2295) (2064 2 pts 3 pts 3 pts 45 n46 A. 1920, 1680 and 761 base pairs long. B. 1920, 1305, 761, and 377 base pairs long. C. 3056, 2295 and 375 base pairs long. D. 2681, 921 and 761 base pairs long. E. The sizes cannot be determined from the data given. n 47 on 48 3 pts items Mar 27 at 00 PM PreviousNext

Explanation / Answer

The resulting fragments would be this large:

A) 1920, 1680 and 761 base pairs long.

2295 - 375 = 1920 bp

(4361 - 3056) + 375 = 1680 bp

3056 - 2295 = 761 bp

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