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Question 11 6 pts You have been given the following viral DNA. Its maps showing

ID: 216795 • Letter: Q

Question

Question 11 6 pts You have been given the following viral DNA. Its maps showing the sites for the restriction enzymes A, B and C are shown below Viral DNAX 5,000 20,000 25,887 (bp) 10,000 15,000 A 853 796 24,372 12,122 4.427 3,215 8,463 18,571 23,518 4,617 525 4, 854 10,121 20,007 24,222 Determine the number of fragments, and their sizes, that would be generated for each enzymes and draw them on the gel below. Indicate the size next to the fragment. 10,000 8,000 6,000 4,000 3,000 2,500 2,000 1,500 1,000 750 500 250 Upload Choose a File

Explanation / Answer

A.)

No. of fragments - 7

Size: 853, 3574, 7695, 6875, 292, 5083, 1515.

B.)

No. of fragments - 6

Size: 3215, 1402, 3846, 10108, 4947, 2369.

C.)

No. of fragments - 6

Size:

525 - 0 = 525,

4854 - 525 = 4329,

10121 - 4854 = 5267,

20007 - 10121 = 9886,

24222 - 20007 = 4215,

25887 - 24222 = 1665.

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