If you can please explain question #6 in the simplest and detailed explanation s

Question

If you can please explain question #6 in the simplest and detailed explanation so that I can understand your reasoning. Thank you

In vivo Methods You decide that dissecting rats and grinding up their livers isn't to your liking, so you're excited to learn that your second lab rotation will involve work with living yeast cells. One of your new colleagues discovered recently that some of her fully engineered strains of yeast cells secrete insulin only at room temperature (25 °C). When they are grown at warmer temperatures (35 °C), they stop secreting insulin and grow poorly; some strains won't grow at all at warm temperatures. Your new mentor asks you to study several of these strains to determine the cause(s) of their insulin secretion defects.

Explanation / Answer

Ans Yeast cell have ability to grow insulin .As it is given above that genetically engineered yeast cell has ability to grow at its maximum only at 25 C and at 35 they are unable to grow and secrete insulin.Now it is asked to study the strain to know the insulin secretion defects.

The thing is the cells are not growing properly at 35.So there can be hypothesis that because the cells are not growing properly they are unable to secrete insulin so the mentor is suggesting that first we should grow the cells properly at 25C and then switch the temperature to see wether still the yeast are able or unable to secrete insulin.If the cells now secrete insulin then it is clear that insulin secretion defect is due to improper growth at 35 C and if the cells still cannot secrete insulin then it is clear that the temperature is not suitable for insulin secretion.

Now the mentor has provided anti insulin antibodies which is cross linked to alkaline phosphatase.This is given so that we can quantify the amount of insulin secreted by yeast cells.Antibodies are highly target specific in their action so they will bind to the insulin molecules.

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