A graduate student wants to visualize mitochondria in live cells. He chooses to

Question

A graduate student wants to visualize mitochondria in live cells. He chooses to use MitoTracker Orange and the fluorescent spectrum is shown below. The dashed line represents excitation curve (peak at 550 nm) and the solid line represents the emission curve (peak at 577 nm). (You can get more detailed information on the website: http://www.thermofisher.com/us/en/home/life- science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html and select "MitoTracker Orange" under "Fluorophore". MitoTracker Orange 100 75 ? 50 25 300 400 500 600 700 800 900 Wavelength (nm)

Explanation / Answer

Ans- 63X water immersion lens will be used for this because it has the highest NA which will be required for a better resolution. Low NA will lead to poor resolution.

Resolution= 1.22lambda / NA of objective + NA of condensor

= 1.22×550/1.5+0.5

=335.5

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