4 23. In the Anthony method of staining the bacterial capsule, which step is not

Question

4 23. In the Anthony method of staining the bacterial capsule, which step is not included in order to avoid shrinking and destroying the capsule a. Smear preparation b. a basic dyec. Heat fixing d. All of the above 24. In the acid fast stain, an organism such as Mycobacterium smegmatis will tain after a successful staining technique. a. Blue due to the primary stain, methylene stain b. Pink to red due to the primary stain, carbol fuchsine c. Green due to the primary stain, malachite green d. None of the above 25. The Schaeffer-Fulton method of staining utilizes _ t stain the endospore and to stain the vegetative cell a. Safranin; malachite green c. Methylene blue; iodine d. Iodine; methylene blue b. Malachite green; safranin 26. A common media used in a Microbiology lab is nutrient agar. It is considered a media because its exact composition is not known but it contains all the necessary ingredients for most facultative organisms to grow successfully a. Defined b. Selective c. Minimal d. Complex 27. Rod shaped bacteria are called a. Bacilli b. Streptococci c. Cocci d. Spirilla in 28. Some bacteria such as Clostridium form a thick- walled structure called response to poor environmental conditions. a. Endospore b. Capsule c. Sheath d. Pilus 29. Nitrifying bacteria are an example of a. Photoautotrophs c. Chemoheterotrophs d. Photoheterotrophs 30. Organisms that obtain their energy by oxidizing inorganic chemical sources are called a. Photoautotrophs c. Chemoheterotrophs d. Photoheterotrophs b. Chemoautotrophs 31. If you carried out an experiment growing different types of microorganisms in liquid culture, which type of organism would you find congregated just a little below the liquid's surface? a. obligate aerobes b. obligate anaerobes c. microaerophiles d. aerotolerant bacteria 32. A particular agent that only inhibits the growth of bacterial cells but does not kill them is said to be while an agent that kills the bacterial cells is said to be a. Antiseptic; bacteriostatic b. Bacteriostatic; bactericidal c. Bactericidal; bacteriostatic d. Sporocidal; fungicidal 33. Which term best describes an organism that can't exist in the presence of oxygen? a. b. facultative aerobe c. obligate anaerobe d. facultative anaerobe obligate aerobe

Explanation / Answer

23. c. Heat fixing.

Heat fixing will destroy the structure of capsule, so this step is avoided in this capsule staining method.

24. b. Pink to red due to primary stain, carbol fuchsin.

Mycobacterium smegmatis is acid-fast positive bacterium.

25. b. Malachite green; safranin

Endospore stains gree while the cell stains pink.

26. d. complex

Nutrient agar is a complex media, the ingredients are not defined here.

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