The two strands of a DNA double helix, dissolved in a pH buffer or water, can be
ID: 217517 • Letter: T
Question
The two strands of a DNA double helix, dissolved in a pH buffer or water, can be separated by heating (denaturation, "melting) 1) 5-GCGGGCCAGCCCGAGTGGGTAGCCCAGG-3 56. 3'-CGCCCGGTCGGGCT CACCCATCGGGTCC-5. 2) 5-ATTATAAAATATTTAGATACTATATTTACAA-3 3-TAATATTTTATAAATCTATGATATAAATGTT-5 3) 5-AGAGCTAGATCGAT-3 y.TCTCGATCTAGCTA-5. If you raised the temperature of a solution containing the three DNA fragments above, in what order do you suppose they would "melt"? Explain your answer a) 1,3,2: fragment 1 has the highest G/C content, it will melt first b) 2,3, 1: fragment 2 has the lowest G/C content, it will melt first. c) 3,2, 1: fragment 2 has fewer hydrogen bonds than 1: 3 is the shortest fragment here d) Melting order can't be determined based on the information provided
Explanation / Answer
Please find the answers below:
Answer 56: Choice C (the ease of melting depends upon the number of hydrogen bonds in the DNA. More are the hydrogen bonds, more is the difficulty in melting them. Since fragment 1 contains more strong and highest number of GC bonds followed by strand 2 and 3, it would be melted in the last)
Answer 63: Choice E (The two strands of DNA are complementary to each other having 3' to 5' orientation in opposite direction. The DNA contains nitrogenous bases where a purine always bonds with only a pyrimidine)
Answer 64: Choice E (RNA has different qualities and properties as compared to DNA and hence, lack of secondary structure is one of them. Specialized RNA molecules exhibit enzymatic properties and they are called ribozymes)
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