Answer all the following 50 questions. Part A: Questions 1-40 (multiple choice)
ID: 217719 • Letter: A
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Answer all the following 50 questions. Part A: Questions 1-40 (multiple choice) on scantron card. Part B: 2 short answer questions to be an Please return scantron cards and questi swered on question sheet on sheet. Time allowed: I hour Part A: Multiple Choice (1-40) 1. Which objective provides the greatest field of view? a.100x b.40x c. 10x d. all provide the same field of view 2. As compared to a light microscope, the resolving power of the electron microscope is a. Greater b. Less c. Equa d. None of the above 3. The total magnification power of a light microscope can be a. Adding the eyepiece magnification plus the objective magnification b. Subtracting the objective magnification from the eyepiece magnification c. Multiplying the objective magnification and the objective magnification d. None of the above attained by 4. To regulate the amount of light that goes through, a student should adjust the a light microscope. a. Condenser b. Eyepiece c. Diaphragm of d. Body tube 5. An organism such as E coli is classified as a Biosafety level a. One c. Three d. Fourorganism, b. Two 6. The practice of containment by Microbiologists a. Prevents workers form contaminating themselves b. .Ensures that others and the work area do not become c. Ensures that microbial cultures do not become contaminated with unwanted organism d. All of the above contaminated 7. Which of the following would be best observed for a bacterial cell a. Motility of the cell b. Bacterial nucleoid c.Cell wall using a phase contrast microscope? d. Glycocalyx b. prokaryotes e. eukaryotes d. responsible for decay of dead plants Te retrieve a sample from a culture tube with an inoculating loop, the cap of the tube a. Removed and held with your teeth b. Removed and held with the fingers of the loop hand c. Removed with the fingers of the loop hand and placed on the lab bench d. Any method above works well a. saprophy 10. A contains only a single kind of organism, where as a culture contains more than one kind of organism. d. Mixed; pure a. Mixed; aseptic b Aseptic; pure c. Pure;, mixedExplanation / Answer
Answer 1. Correct option is C (10X). Higher the magnification lower will be the field of view.
Answer 2. Correct option is A (Greater). Limit of resolution for light microscope is 200 nm whereas the limit of resolution for electrom microscope is 0.1 nm.
Answer 3. Correct option is D (none of the above). To calculate the total magnification power of a microscope we need to multiply the magnification power of objective lens with the magnification power of eyepiece.
Answer 4. Correct option is C (Diaphragm). It is usually located above the condenser and below the stage.
Answer 5. Correct option is A (One). Non pathogenic strains of E.Coli are typically worked with at BSL safety level 1.
Answer 6. Correct answer is D (All of the above).
Answer 7. Correct answer is C (Cell wall). Phase contrast microscope solves the problem to see unstained cells and bacteria.
Answer 8. Correct option is B (Prokaryotes). Fungi are eukaryotic organisms. They are saprophytic in nature.
Answer 9. Correct option is B (Removed and held with finger of loop). This is the basic rule of good laboratory practice.
Answer 10. Correct option is C (Pure, Mixed). Pure culture has single organism and mixed culture include 2 or more organisms present.
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