Answer all the following 50 questions. Part A: Questions 1-40 (multiple choice)

Question

Answer all the following 50 questions. Part A: Questions 1-40 (multiple choice) on scantron card Part B: 2 short answer questions to be answered on question sheet Please return scantron cards and question sheet. Time allowed: I hour Part A: Multiple Choice (1-40) 1. Which objective provides the greatest field of view a.100x b.40x c. 10x d. all provide the same field of view 2. As compared to a light microsecope, the resolving power of the electroe microscope is a. Greater b. Less c. Equald. None of the above 3. The total magnification power of a light microscope can be atained by a. Adding the eyepiece magnification plus the objective magnification b. Subtracting the objective magnification from the eyepiese magnificatione d. None of the above 4. To regulate the amount of light that goes through, a studet should adjust the of light microscope a. Condenser b. Eyepiece e.Diaphragm d. Body tube 5. An organism such as E col is classified as a Biosafety levelorganism a. One b, Two c. Three d. Four 6. The practice of containment by Microbiologists a. Prevents workers form contaminating themselves b. .Ensures that others and the work area do not become contaminated c. Ensures that microbial cultures do not become contaminated with unwanted organism d. All of the above 7. Which of the following would be best observed for a bacterial cell using a phase coetrast microscope? a. Motility of the cell b. Bacterial nucleoid c. Cell wall d. Glycocalyx s. Fungi ne all the following except are asexual fungal spores except a. saprophýtes b. prokaryotes c.eukaryotes d. responsible for decay of dead plants To retrieve a sample from a culture tube with an inoculating loop, the cap of the tube a. Removed and held with your teeth b. Removed and held with the fingers of the loop hand c. Removed with the fingers of the loop hand and placed on the lab bench d. Any method above works well 9. 10. A a Mixed: aseplicbAseptier pure e.ur micde culture contains more than one kind of organism. Mixed, pure

Explanation / Answer

1.c 10x. Field of view means how much of your specimen or object you will be able to see through microscope. In all these options10x has lowest magnification and highest field of view.

2. Greater as electron microscope can magnify up to 10000000x while light microscope has 2000x magnification.

3. By multiplying objective magnification and the eye piece magnification.

4. Diaphragm as it controls the intensity of light entering the lens system.

5 (one) because non pathogenic strain of E. Coli is BSL level_1

6. All of the above.

7 Bacterial nucleoid. It is used to see cell organelles without staining.

8. Prokaryotes. Fungi are not prokaryotes

9 removed and held with finger by loop hands.

10 c.pure mixed. A pure culture contains only a single type of microorganisms where as a mixed culture contains more than one types of microorganism

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