Short Answer. A recently isolated bacterial strain has been given to you for gen

Question

Short Answer. A recently isolated bacterial strain has been given to you for general characterization of the genome. You begin by isolating the DNA and subjecting it to thermal denaturation. You use the common bacteria Escherichia coli DNA as a reference. 1.41 1.2- 1.0 70 80 90 100 Temperature C) -Ecoliunknown organism Based on the information in the graph, which organism do you think has a higher GC content and why? Think about the number of hydrogen bonds formed between GC pairs versus AT pairs; and the different amount of energy needed to break them.

Explanation / Answer

Answer:

Unknown organism will have high amount of GC pairs than AT pairs so that they are more stable even at higher temperatures.

AT pairs are held toghether by two hydrogen bonds whereas GC pairs are held together by three hydrogen bonds. To break three hydrogen bonds, more amount of energy is needed. Hence, the organism that contains more GC pairs are more stable. Based on the graphical information, the unknown organism has higher GC pairs and is stable at higher temperature.

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