sapps-Swekyb3d8bbwe/LocalState/Files/SO/4862/Bio%20101%20at E. Effect of pH on E
ID: 218596 • Letter: S
Question
sapps-Swekyb3d8bbwe/LocalState/Files/SO/4862/Bio%20101%20at E. Effect of pH on Enzymatic Activity Peroxidase activity will be determined at different Ht ion concentrations. 1. Number 6 clean spectrophotometer tubes, I through6. 2. Set up the reactions according to the table below using the bulfers provided (do not use the PBS buffer). USE 33 ml of THE SPECIFIED BUFFER PER TUBE. (Use a different 5 ml pipette for each buffer) VORTEX each tube when ALL substances have been added. BUFFERml ENZYME ml OPD ml PEROXIDE A 410 nm 0.1 0.1 TUBE PH 5 pH 5 pH 7 0.1 0 0.1 0 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 pH 9 pH 9 0.1 3. Incubate all tubes at room temperature for 15 minutes and read the absorbency at 410 nm. Use test tube #1 to blank the spectrophotometer. (MIX EACH TUBE ON THE VORTEX MIXER BEFORE DETERMINING ABSORBENCY) 4. Why did you set up tubes 1,3, and 5 5. At which pH did the enzyme catalyze the reaction most efficiently?Explanation / Answer
Answer
4. 1,3,5 are used as a control in this experiment. With out enzyme what is the absornance done by the reactants are measured. It like blank used in normal experiments.
5. At pH 7 it shows optimum activity.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.