eene for insulin has been inserted into a vector for the purpose of obtaining it

Question

eene for insulin has been inserted into a vector for the purpose of obtaining its protein product only Such a vector is called a expression vector storage vector for genomic library d. none of the above 23. Which of the following is NOT true about a cDNA Library? a A cDNA library can be used to clone any piece of DNA from the organism b A cDNA library only contains sequences that were expressed as mRNA in the tissue from which the original RNA was isolated c. Onily active genes are included d. cDNA library includes intron 24. You discover a novel EUKARYOTIC organism that glows in the dark. You believe this trait is due to a single gene, and you wish to clone the gene. Which of the following strategies is most likely to be successful? Isolate the genomic DNA from the organism, digest with a restriction endonuclease, insert into a plasmid vector and transform into bacteriA. Screen colonies for the ability to glow in the dark Isolate the genomic DNA from the organism, digest with a restriction endonuclease, insert into a plasmid vector and transform into eukaryotic cells such as yeast. Screen colonies for the ability to glow in the dark Isolate mRNA from the organism, reverse transcribe and generate cDNA, insert into a plasmid vector and transform into bacteriA. Screen colonies for the ability to glow in the dark Isolate mRNA from the organism, reverse transcribe and generate cDNA, insert into a plasmid vector and transform into eukaryotic cells such as yeast. Screen colonies for the ability to glow in the dark a. b. d. 25 Which of the following would you expect to be a drawback to PCR techniques? a. PCR has the potential to create positive results from very low levels of contamination b. PCR can be used with very small samples such as single sperm cells C. PCR is very rapid; in some cases results can be available in a few hours. d. All of these 26. In choosing a vector, viral vectors typically can carry much larger pieces of DNA than plasmid vectors, but plasmid vectors are much easier to work with since they do not kill their host cells. In which of the following situations would you anticipate preferring a viral vector? a. You need to clone an entire gene, including regulatory sequences. b. You are generating a genomic library from an organism with a large genome C. You are creating an mRNA library to identify the gene encoding a very large protein. d. All of these e. None of these. 27. Electroporation is: a the process of separating charged molecules through a gel maintained in an electric field b. the process of combining foreign DNA to an electrically charged vector molecule c. the process of introducing DNA into cells by the application of high voltage pulses d. All of the above

Explanation / Answer

1) Expression vector - Expression vectors are vectors that are used to produce proteins, which had been coded by the corresponding genes. Vectors can also be used to store genes and form genomic libraries. There are no suppressor vectors.

2) cDNA library includes intron - The cDNA library consists of only exons, because the mRNA is transcribed only from exons, not from introns. When all of the sequence is stored as cDNA libtrary, any piece of DNA from the organism can be cloned.

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