Imagine you have performed a de novo assembly of the genome of a newly isolated

Question

Imagine you have performed a de novo assembly of the genome of a newly isolated bacterium using Illumina. You have 100 contigs (contig = continuous piece of assembled sequence), totaling 3.5 Megabase pairs in length. Based on the size of the genomes of related bacteria, it is probable that you are only missing 150 kilobase pairs of sequence. Usually, gaps are due to repetitive sequences. When short-read length technologies are used, the alignment software becomes confused by the repeats.

In a circular bacterial genome, 100 contigs means 100 gaps. You expect that 150 kbp of sequence is missing, but you can't be sure and you can't know how big the gaps are.

Which of the 4 sequencing technologies would you choose to try to finish sequencing the genome? Why?

Explanation / Answer

ANS) For 4 sequencing technologies i would choose PacBio to finish sequencing the genome,

Because PacBio SMRT frameworks doesn't undergo with any pre-intensification ventures as these frameworks are sufficiently sensitive to recognize single particle expansions. The distinctive methodologies hence can read contrasts with regards to the diverse stages.

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