Types of microscopy discussed in class: 1. Fluorescence microscopy 2. Confocal s

Question

Types of microscopy discussed in class: 1. Fluorescence microscopy 2. Confocal scanning microscopy 3. Transmission electron microscopy
Which of the microscopic techniques discussed in the course would you use for observation of each of the following systems? (list all techniques that apply) a) Microbe cells in water? b) Liquid crystal droplets in water? c) Thin microfluidic channel etched in transparent plastic? d Following the trajectory of a single particle in dense suspension of other particles? e) Resolving structure of three-dimensional aggregates of particles? f Real-time observation of nucleus division inside live cells? g) Measuring shape and diameter of nanometer sized metal particles? h) Visualizing areas of oppositely charged thiols in a patterned SAM on gold?

Explanation / Answer

a. Microbe cells in water - Fluorescence microscopy.

Reason: Cells can be visualized using a fluorescent stain and a slide.

b. Liquid crystal droplets in water - Confocal scanning microscope

Reason: 3D structure of crystal can be visualized using this technique

c. Thin microfluidic channel etched in transparent plastic - Confocal microscopy

Reason: Moven of fluid in XYZ directions can be visualized easily in this microscopic technique

d. Fluorescence microscopy

Reason: Single particle can be labeled using a fluorophore. This will help to follow the trajectory of a single particle in dense suspension of other particles.

e. Scanning electron microscope

Reason: SEM gives highest resolution

f. Fluorescence microscopy

Reason: Using fluorescent tagged proteins, we can visualize the different steps of cell division in live cells.

g. Transmission electron microscopy

Reason: This gives the cross-sectional view of an object with high resolution. It helps to measure the diameter of very small metal particles.

h. Transmission electron microscopy

Self-assembled monolayers are formed by thiols on a gold surface. By taking a cross-section of these layers, we can visualize the areas of oppositely charged thiols.

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