You have a bacterial culture solution. When you directly measured the OD600, of

Question

You have a bacterial culture solution. When you directly measured the OD600, of the culture solution, it was 3. Then, you performed a 10-fold dilution and measured the OD600

of the diluted solution, which was 0.5. Thirdly, you performed another 10-fold dilution and measured the OD600 of the diluted solution, which was 0.07. Assuming that OD

600 =1 is equivalent to 10^9 cells/ml, how many cells do you have in 1ml of the original culture?

You have a bacterial culture solution. When you directly measured the OD6o0, of the culture solution, it was 3. Then, you performed a 10-fold dilution and measured the OD6o0 of the diluted solution, which was 0.5. Thirdly, you performed another 10-fold dilution and measured the OD600 of the diluted solution, which was 0.07. Assuming that OD6o0-1 is equivalent to 109 cells/ml, how many cells do you have in 1 ml of the original culture? A) 3 x 109 cells B) 6 x 10 cells C) 7 x 109 cells D) 8x 109 cells E)3 x 1010 cells

Explanation / Answer

The answer is C

It has 7 ×109 cells per ml. The dilution of sample for OD measurement gives correct idea of OD value of solution. If the OD is more than one we must dilute the solution.

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