Put the following steps for the bacterial transformation lab in the correct orde

Question

Put the following steps for the bacterial transformation lab in the correct order Streak 0.25 ml of cell suspension of 3 platen 12 from rube #1 and 1 from tube #2) Mix control butter and ceils in labeled tube #1 and mix plasmid DNA and cells in labeled tube #2 To each tube add 0.75 ml of recovery broth Incubate both lubes at 42 degrees Celsius for 90 seconds Incubate plates overnight in 37 degrees Celsius incubator Place both tubes on ice for 1 minute incubate both tubes 37 degree, Celsius water bath for 30 minutes incubate both tubes on ice for 10 minutes.

Explanation / Answer

steps in order-

1) To each tube ,add 0.75ml of recovery broth.

2) Incubate both tubeson ice for 10 minutes.

3) Mix control buffer and cells in labelled tube 1 and mix plasmid DNA & cells in labelled tube 2

4)  Incubate both tubes at 42 degrees for 90 seconds

5)place both tubes on ice for 1 minute.

6) Incubate both tubes in 37 degrees Celsius water bath for 30 minutes

7)Steak 0.25ml suspension of 3 plates.

8)Incubate plates overnight in 37degrees celsius incubater.

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