46-……e- aaa.m- wi-tcas hen-wed mk etheteoffire ers Prulains which ofehe folowing

Question

46-……e- aaa.m- wi-tcas hen-wed mk etheteoffire ers Prulains which ofehe folowing is the best by adding EPTG to turn expression of the lacZ geometer level of brtese mat is added to the gr median b.pTmoars are co are controlled by how meh arabi-isadied tothe grwes meduus. dpTvetrs are coeeruladby much evye ayan added te the growth medium. 17 The stabdity of recenhinantproteins inside eels can be improved by n of molecular chagerenes b. A eration of the N tennial amino acid. of PEST sequences d Allot the above. e. Some of the above. 28. Problems in an producing secretary proteins include all of the following EXCEPT a The protein may be secreted into the periplasmic space rather than outside the cell. b. it is far more difficult to purify proteins from the growth medium than from inside the cell. c Genes can be expressed in Bacillus which doe s secrete into the culture medium, but the genetics isnot as well d All of the above are problems secreting proteins. Some ofthe above are problems secreting proteins. 19-All of the following are examples of posttranslational modifications EXCEPT Glycosylat ion such as found on cell surface proteins. b. Addition of groups such a s acetyL phosphate, sulfate, and fat acid chains. C. The addition of leader sequences toescort proteins outside of the cell d C hemical modification to form novel amino acids. e, formation of disunde bonds. 20. Yeast are excellent choices for expression of eukaryoti e proteins for all of the following reasons EXCEPT EXCEPT a. Like E. coli, y east are easy and quick to grow. b. Yeast are used in food production, thus are considered safe.

Explanation / Answer

19.

Most of the proteins that are translated from mRNA undergo chemical and enzymatic modifications during or after protein synthesis before becoming functional in different cells. These chemical and enzymatic modification are known as post-translational modifications.

It may occur in several ways for example:

A. Glycosylation : Addition of carbohydrates

B. Acetylation : Addition of acteyl group at N-terminus of protein

C. Alkylation : Addition of alkyl group

D. Methylation: Addition of methyl group usually at lysine or argining residue.

E. Phosporylation: Addition of phosphate group

F. Sulfation: Addition of sulfate group

So all option a,b,d, e are examples of post-translational modification

The answer is option C.

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