You have sequenced a short piece of DNA and produced the gel shown. A. What is t

Question

You have sequenced a short piece of DNA and produced the gel shown.

A.        What is the sequence of the DNA, starting from the 5 end?

B.        If you knew that this sequence is from the middle of a protein-coding cDNA clone, what amino acid sequence can be deduced from this sequence?

(A ddT ddA ddC ddG (B) The Genetic Code UU Phe (F) UCU Ser (S) UAU Tyr (Y) UGU Cys (C) UUC UCC UAC UGC UUA Leu (L) UCA UAA Stop UGA Stop UUG UCG UAG Stop UGG Trp (W) CUU Leu (L) CCU Pro (P) CAU His (H) CGU Arg (R) CGC CUC CAC CUA CCA CAA Gln (Q) CGA CGG CUG CCG CAG AUU Ile (l) ACU Thr (T AAU Asn (N) AGU Ser (S) AUC ACC AAC AGC ACA AAA Lys (K) AGA Arg (R) AUA AGG AUG Met (M) ACG AAG GU Val (V GCU Ala (A) GAU Asp (D) GGU Gly (G) GUC GGC GCC GAC GAA Glu (E) GGA GCA GUA GCG GUG GAG tb 10-06

Explanation / Answer

(A) 5'TAGACTGACCTC3'

(B) mRNA is =3'- AUC UGA CUG GAG-5'

mRNA is read in 5'-->3' direction

the 5' to 3' mRNA is= 5'-GAG GUC AGU CUA-3'

peptide formed = Glu-Val-Ser-Leu

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