Which of the added followed by a PAGE is nin followed by a with a and an blotted

Question

Which of the added followed by a PAGE is nin followed by a with a and an blotted to analyzed. s AMALDIisa alas speceremetry technique allows very large molecules such as proteins to be the ions that ionized and a ugud containing proteins are metal bar. The protein is solvent evaporates After heating the Proteins tube and detected TOF, proteins to lose by and ions are released into the vacuum Laser energy then causes c Proteins embedded into 2 mic acid bons move through vacuum and be detected by TOF. d None of describe MALDI In which of the following ways can mass spectrometry be used to determine the sequence of large a Protein in extracts are digested with proteases and then separated by SDS-PAGE. Individual peptides are seguenced by mass spectrometry and then the sequence is pieced back togethe

Explanation / Answer

5) Answer D : None of the above describe MALDI

MALDI TOF - Matrix Assisted Laser Desorption Ionization Time-of-Flight (TOF) mass spectrometry is a soft ionization technique used in mass spectrometry (MS) used to analyse and determine the mass of biomolecules especially peptides and proteins. The sample is embedded in the matrix and when laser is passed on, protein or peptide get ionized to become protonated or deprotonated. Then it is detected by TOF to determine the mass.

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