I don\'t need all of the data to be done for me, just a quick how to solve will

Question

I don't need all of the data to be done for me, just a quick how to solve will work. Thank you in advance!

When self-complementary strands of DNA are mixed, they form a double-stranded DNA at low temperatures but dissociate to single strands as the temperature is raised: This can be observed by an increase in the absorbance of the solutions at 260 nm (The double helix is said to show "hypochromicity" at this wavelength) Data for two experiments are given on the right. In one case, short DNA strands made of A and T nucleotides (oligoA-T) were mixed, whereas, in the other case, the same length strands and concentrations of oligomers of G and C (oligoG-C) G were mixed. a. Assume that the molecules are duplexes (D) at the lowest temperatures for which data are given and single strands (S) at the highest temperatures. Calculate the fraction present as duplex at each temperature.

Explanation / Answer

Strands in lowest temperature remain duplex therefore; we would consider the lowest temperature Optical density as standard. Now, we can calculate the fraction present as duplex like given below.

Fraction of duplex = 0.711/ O.D. at T [where T is temperature ]

For example at 100 C, Fraction of duplex = 0.711/ 0.720 = 0.99

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