Weed control is a concern in both agriculture and ecology, and scientists are al

Question

Weed control is a concern in both agriculture and ecology, and scientists are always testing new herbicides in order to try to improve crop yields and eradicate invasive species without damaging the environment. Before being used, however, one must determine an herbicide's mechanism of action. If you wanted to test whether, like DCMU, a new herbicide 'steals' electrons from the Hill Reaction, preventing them from entering the chloroplasts' electron transport system

A. Done properly, this experiment would incude both negative and positive controls. What would you use for each of these controls?

positive and negative controls

B. Shown in the table at right is data from a hypothetical experiment testing the mechanism of a new herbicide (herbicide X or HCX) using the same methodology we used in Part 3. Does this herbicide 'steal' electrons from the Hill Reaction like DCMU? If so explain how you know. If not, explain why not.

HCX

"low"

HCX

"high"

0

10

1.07

0.45

1.09

0.88

1.07

0.44

1.08

1.07

Time (min) A600

HCX

"low"

HCX

"high"

negative control positive control

0

10

1.07

0.45

1.09

0.88

1.07

0.44

1.08

1.07

20 0.44 0.72 0.44 1.07

Explanation / Answer

I would use the solvent/ carrier alone for negative control and DCMU as positive control. This herbicide X or HCX don’t 'steal' electrons from the Hill Reaction like DCMU as increasing the dose from low to high the value increased from 0.45-0.88 i.e. the difference from initial was reduced. Whereas in positive control 1.07 and negative control it is 0.44

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