The yeast Saccharomyces cerevisiae can grow as haploid or diploid cells. You hav

Question

The yeast Saccharomyces cerevisiae can grow as haploid or diploid cells. You have two haploid yeast strains that each carry mutations that make them unable to grow when galactose is the sole available source of energy. One strain has a deletion of the entire region of the genome between the GAL1 and GAL10 genes (DUAS). The other strain carries a mutant allele of GAL2 that produces no functional GAL2 protein. You cross these two strains together to get diploid progeny. Will these diploid cells be able to grow on media where galactose is the only source of energy

Explanation / Answer

Generally, wild-type yeast cells can grow on variety of carbon sources other than glucose to support growth. In particular, raffinose and galactose are used under conditions to relieve glucose repression (in the case of raffinose) or to induce expression from a Gal4p-dependent promoter such as GAL1 and GAL10. In the above experiment, the crossed diploid cells will not grow on media where glactose is the only source of energy because nne has an inactive GAL10 gene and the other can’t activate it’s GAL10 gene.

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