I was given a protein sample to determine its sequence. Here are the steps I fol

Question

I was given a protein sample to determine its sequence. Here are the steps I followed 1. I did an amino acid analysis and got the following Results of the amino acid analysis 5 H2 38 amino acids total D 4 V 1 M 2 Y 2 G 3 2. From the amino acid analysis, I determined that Trypsin and CNBr could be used as two protein cleavage agents to generate smaller fragments of the protein. Think about how did I make that determination? 3. Next, I purified each fragment (for each set of digestions) and sequenced them by Edman's method T-1 GASMALIK T-2EGAAYHDFEPIDPR T-3 DCVHSD T-4 YLIACGPMTK Results of sequencing of peptides obtained from CNBr digestion C-1 EGAAYHDFEPIDPRGASM C-2 TKDCVHSD C-3 ALIKYLIACGPM 4. Help me align the overlapping fragments from the two sets of digestions to know the sequence of the protein!

Explanation / Answer

Cyanogen bromide hydrolyzes peptide bonds at the C-terminus of methionine residue

these two enzymes might have been chosen because they have lesser number of cleavage sites

Trypsin cleaves peptide chains mainly at the carboxyl side of the amino acids lysine or arginine.

EGAAYHDFEPIDPRGASM ALIKYLIACGPM TKDCVHSD

C-1 C-3 C-2

Related Questions

Navigate

• Browse (All)
• Browse I
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.