A microbiology student was carrying out an independent research project about th

Question

A microbiology student was carrying out an independent research project about the bacteriophage, P2vir. As part of the project, the student was provided with a tube of pure, filtered P2vir to use as a stock solution for several experiments. Recently, a plaque assay had shown that a 0.1 mL sample taken from a 10-3 dilution of this stock solution generated 98 plaques. To begin an experiment one day, the student took a P-20 pipetteman, dialed the setting to match the setting shown here, and withdrew this volume from the P2vir stock solution tube.

Explanation / Answer

100 µl of stock solution contains 98 X 103 plaques.

So, 5 µl of stock solution contains 98 / 5 X 103 plaques

That is 19.60 X 103 PFU

The student removes 19.60 X 103 PFU from the stock tube.

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