A microbiology is working on delivering nutritional anabolic genes to auxotrophi

Question

A microbiology is working on delivering nutritional anabolic genes to auxotrophic mutants which are defective in three amino add synthesis processes (genotype of the autotrophic mutant trp, tyr-, hfs-). After gene delivery, some bacterial colonies can now grow on a certain defined nutritional medium, given in the questions below. Please identify what genes that they have acquired to grow, using the choices from #a to #f a. Tyr^+ revertants b. His^+ revertants c. Try^+ revertants d. Tyr^+, Trp^+ revertants e. Tyr^+, His^+ revertants f. Trp^+, His^+ revertants The bacterial colonies can now grow in minimal nutritional agar plates with Tyrosine and Histidine amino acids added The bacterial colonies can now grow in minimal nutritional agar plate, with Tyrosine and Tryptophan ammo acids added The bacterial colonic can now grow in minimal nutritional agar plates with Trp amino acid added. A microbiologist delivered his target gene via a viral T4 phage transduction system. This target gene is cloned into a vector containing an Amp^+ drug resistance market. Please answer the following questions to help this microbiologist set up the experiments and screen for positive transductants. What type of growth medium should the microbiologist use to select for positive transductants? a. LB/Kanamycin plate b. LB plate c. LB./Ampicillin broth d. LB/Ampicillin plate e. None of the above On the proper selective plates, 50 colonies grew on a "1:100" dilution plate. Estimate around how many colonies would be seen on a -1:10" dilution plate?

Explanation / Answer

Since three anabolic nutritional genes are delivering in to the mutants which are defective in those three amino acid synthesis. So, on the basis of these information find the answers of the question below:-

16. If the bacterial colonies can grow on the media containing tyrosine and histidine amino acids, the answer should be (e), try+, His+ revertants.

17. The answer should be (d), try+, Trp+ revertants.

18. The answer should be (c), Trp+ revertants.

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