A biochemistry laboratory student used the Bradford protein assay to measure the

Question

A biochemistry laboratory student used the Bradford protein assay to measure the lysozyme (protein) content in egg whites. The Bradford protein assay is a spectrophotometric technique that uses a dye, Coomassie Brilliant Blue, whose absorption undergoes a spectroscopic shift when bound to a protein. In the unbound state, Coomassie Brilliant Blue displays a red color. As protein binds to the dye, its absorbance at 595 nm increases and the dye changes to a blue color. The student prepared a 3.200 mL sample containing 2 mg/mL Coomassie Brilliant Blue. To this sample, 500.0 uL of 35.0 ug/mL lysozyme was added and an absorbance of 0.326 was measured at 595 nm. Calculate the corrected absorbance for this sample. Number Corrected absorbance-

Explanation / Answer

Corrected absorbance= blank absorbance-individual absorbance.

C A = 0-0.326.

= -0.326

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