A bioengineer is running fermentation experiments to produce a new biofuel. The

Question

A bioengineer is running fermentation experiments to produce a new biofuel. The biofuel is produce as a byproduct of normal metabolism of lactose by a newly identified fecal coliform bacterial species. Currently the yield of biofuel is half the expected amount. The engineer suspects that there may be another bacterial species contaminating the recent fermentation. Use the information below to answer the following: Design an experiment using only what we have learned in class to determine A) If there is another bacterial species contaminating the fermentation and B) how to determine if the contaminating bacterium is affecting the biofuel yield.

Explanation / Answer

Answer:

To test that if another bacterial species is contaminating the fermentation the below test can be performed:

- biochemical test with appropriate reagents

- Microbial contamination and identification test

- Metabolite production test

- Culture plate method to identify contaminants

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