Question
Hi. I'm working on my biology report.
This experiment is Measurement of Catalase Activity using Yeast Spheres.
Can you answer the questions below?
I would appreciate :)
Introduction to Biology and Lab- BIO105 Measurement of Catalase Activity using Yeast Spheres Introduction Catalase, found in both plant and animal cells, has the extremely important function of preventing the accume of toxic levels of the strong oxidizing agent hydrogen peroxide (H202), a by-product of metabolic processes. Be this enzyme is so ubiquitous it is frequently used in high school biology laboratories to explore enzyme reaction One common method is to dip paper disks in a yeast or potato solution and place the disks in a container of hydrogen peroxide. The disks initially sink but as catalase reacts with the H202, O2 is produced and the disks rise 2H202 catalase ->2H20+02 While this is an easy experiment to perform there are some variables that are difficult to control (e.g. the amoun yeast or potato solution on the disk). This workshop will explore an equally easy and perhaps more enjoyable way do the same experiment that reduces the inherent variability of the paper disk design. Yeast cells are encapsulate in sodium alginate, a non-toxic, easily obtainable, algal extract, to form uniform spheres. These spheres are dropp into H:02 and the time for the spheres to rise is measured. The potential for inquiry-based experiments abound; what happens if the temperature is changed, pH, H202 concentration? Because the reaction is fairly quick, multiple replicates can be performed in a short amount of time so statistics may be used to interpret the data. Procedure: Part I Making Beads Basic Procedure Preparing the yeast/sodium alginate solution 1. Add equal volumes of a 10% solution is very viscous). Mix well with a glass rod. (20 ml of each, mix with end of inoculating loop) yeast (Saccharomyces cerevisiae) solution to a 2% sodium alginate solution (this -sodium alginate solution into a 30 ml syringe. Carefully wipe off all excess liquid from the 2. Draw up the yeast syringe tip Making the yeast spheres 1. Hold the syringe containing the yeast -sodium alginate solution over a beaker r containing 50 ml of 0.15M CaCla. held over plastic cup about 1/3 full with 0.15M CaCla). Very slowly depress the plunger so that a drop of sodium alginate solution falls into the beaker. A sphere should form as the drop solution and fall to the bottom of the beaker t with the CaCla 2. Continue releasing yeast-sodium alginate drops into the 0. sze. The spheres should remain in this solution for about 5 minutes to harden. 15M CaClz solution. Try to have spheres all of uniform
Explanation / Answer
ANSWER 1)
1A) catalase
1B) Hydrogen peroxide or h202 is the substrate
1C) product is 2 molecules of water per hydrogen peroxide molecule.
1D) O2 is produced as a gas and cen be demonstrated by the presence of bubbles during the reaction.
2) as the enzyme concentration is increased, the activity of the enzyme will increase as more enzyme will be simultaneously converted from substrate to product but to a limit where substrate concentration acts as a limiting factor after which increasing enzyme concentration will have no effect.
3) the rate of enzyme activity will again increase in substrate concentration is increased as more substrate is available for enzyme.
Feel free to leave a comment down below for any further query. Thank you.
