QUESTION 14 You have a sample which has in i 18,000 bacteria per miL Which of th
ID: 252522 • Letter: Q
Question
QUESTION 14 You have a sample which has in i 18,000 bacteria per miL Which of the following operations would give you a plate containing between 30-300 - A plate 0.1 mL of a 1/100 dilution 0 B plate 1 0 ml of a 1/100 dilution @ ? plate 1 mL of a 1/1000 dilution O D plate 0.1 ml of a 1/1000 dilution O E plate 0 1 ml directly from the original sample colonies? QUESTION 15 Which of the following enumeration techniques results in the largest confidence interval (ile the least confidence)? A pour plate B spread plate C Most Probable Number (MPN) D membrane filtration E none of the above, they all have equal confidence intervals QUESTION 16 A disadvantage of the spread piate technique is that - A it can't be sued to count bacteria that are Bensilive to temperatures above #2 degrees centigrade (Celsius) B only a small inaculation volume can be used C facultative anaerobes won't grow using this t D ia can't be used as an isolation technique E at of the above echnique s. Save All AnsExplanation / Answer
14.
Correct: the colony forming units are calculated by dividing the original sample CFU with the dilution factor. So according to the given case if we take 0.1 ml of the 1/100 dilution than we can get the desired CFU/ml.
Hence the correct option is (A) and other are incorrect as they do not justify the situation.
15.
Correct: most probable number is the technique that give around 95% confidence limit or the larest confidence interval.
Hence, the correct option is (C) and other are incorrect.
16.
Correct: the major disadvantage is that only a small inoculation volume can be used because it would not soaken well and may result to coalesce as they form.
Hence, the correct option is (B) and all other options are the advantages of this technique.
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