Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpos

Question

Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a) necessary digestion of their own genome b) defense against viral infection c) bacteria never make endonucleases naturally 2. A blunt cutter produces DNA fragments with complementary overhanging regions. a) True b) False 3. Which of the following DNA sequences(when double-stranded) most likely represents a restriction endonuclease site for a 6-mer cutter? a) AGTAAGCTTC c) AGAGAGCCAA b) GGTAGATTCC d) None of the above loning vectors have three essential components that make up their sequence. Which one is NOT one of those three? a) origin of replication b) dominant selectable marker d) polylinker region c) polyA region 5. Oligo (dT) and reverse transcriptase are used when making: a) a genomic library b) a cDNA library c) a restriction endonuclease map d) a DNA sequencing reaction 6. 2,3-Dideoxyribonucleoside triphophates (poisoned dNTPs') are used when making: a) a genomic library b) a cDNA library c) a restriction endonuclease map d) a DNA sequencing reaction 7. What type of hybridization results from running genomic DNA on an agarose gel transferring to a membrane, and probing the membrane with a labeled cDNA? a) northern blot b) Southern blot d) eastern blot c) western blot 8. What do we call a small nucleotide polymorphism that results in the production or elimination of a restriction endonuclease site? a) VNTR b) contig e) GFP c) paralogue d) RFLP 9. Coding DNA regions (exons) make up about what percentage of the human e) 75% genome? a) 1% b) 5% c)10% d) 25%

Explanation / Answer

1. defence against viral infection

2.False

3.AGTAAGCTTC

4. poly A region

5. cDNA

6. a DNA sequencing reaction

7. Southern blot

8.RFLP

9. 1.1%

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