Assume you have a solution containing the pyruvate dehydrogenase complex and all

Question

Assume you have a solution containing the pyruvate dehydrogenase complex and all of the enzymes of the TCA cycle, but none of the metabolic intermediates. When you supplement this solution with 5 ?moles each of pyruvate, oxaloacetate, coenzyme A, NAD, FAD, GDP, and Pi, you find that CO2 evolves for a short time, but then stops. (5 points) Previous versions of this question incorrectly stated: "Addition of alcohol dehydrogenase and excess acetaldehyde to your mixture restarted the reaction." However, this is wouldn't work. Explain what the addition of alcohol dehydrogenase and acetaldehyde would accomplish. (5 points) What compound, in addition to alcohol dehydrogenase and acetaldehyde, would you need to add in order to restart your reaction? Explain why this additional compound would restart the reaction. (3 points) what is the maximum yield, in terms of CO2 produced, that your now correctly supplemented mixture can produce? a. b. c.

Explanation / Answer

Ans-A acetldehyde is a product of alcohol digestion. So when we add excess of alcohol dehydrogenase and acetldehyde, the reaction reach to equilibrium because in that case the substrate and the product level become equal after a while due to which the reaction is seized.

Ans-B to restart the reaction we have to add more amount of alcohol as it will lead to increase in the amount of substrate and the equilibrium will not be maintained and the reaction will restart.

Ans-C the maximum yield will depend on the concentration of the alcohol added. From one mole of glucose two moles of each etanol and Co2 are produced.

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