You just completed a transformation protocol with the LM?? strain of Escherichia

Question

You just completed a transformation protocol with the LM?? strain of Escherichia coli, and the pTer plasmid. Cell LMNS2 is a strain of E. coli that has been made competent by the inclusion of a salt in an artificial competency protocol. Name the salt used: LMNS2 is naturally resistant to Nalidixic Acid due to chromosomal Naladixic Acid (1 pt). Plasmid: pTer contains the following: ORi pTer GF Ampicillin" Describe the expected growth on each of the six plates below. Describe what you would see and if there is growth, indicate why the growth was able to occur on the plate (specify where the resistance is coming from-chromosomal or plasmid) (6 pts). LB + LB Amp+ LB Naladixic Acid+ LB LB Amp LB Naladixic Acid

Explanation / Answer

Calcium chloride is the salt used for artificial competence.

Name of the salt: Calcium chloride

LB is required to grow both tranformed anf non-transformed bacteria. Resistance to Amp comes from the presence of plasmid. Ao only the transformed bacteria are resistant ot Amp. Nalaxic acid resistance comes form the chromosome. So both tranformed anf non-transformed bacteria.

LB+: Both transformed and non-transformed bacteria.

LB Amp+: Only trasformed bacteria

LB Naladixic acid+: Both transformed and non-transformed bacteria.

LB-: None of the bacteria would grow.

LB Amp-: Both transformed and non-transformed bacteria.

LB Naladixic acid-: Both transformed and non-transformed bacteria.

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