You just completed a transformation protocol with the ???? strain of Escherichia

Question

You just completed a transformation protocol with the ???? strain of Escherichia coli, and the pTer plasmid. LMNI2 is a strain of E. colf that has been made competent by the inclusion of a salt in an artificial competency protocol Name the salt used: Colcum chlhndk1 pt). LM ?? is naturally resistant to Nalidixic Acid due to chromosomal Naladixic Acid. Cell: Plasmid: PTer contains the following ORi pTer GF Ampicillin* Describe the expected growth on each of the six plates below. Describe what you would see and if there is growth, indicate why the growth was able to occur on the plate (specify where the resistance is coming from-chromosomal or plasmid) (6 pts). Transformed Untronsfor TranSforhed lawn No resist LB Amp+ LB Naladixic Acid + formtd,green,coionies ance fPom piasmid NO Growth LB Naladixic Acid - LB B Amp- Page 1 of 2

Explanation / Answer

LB+, transformed as well as non-transformed, both cells will grow. In addition, contaminant microbes may also be present. The colonies will be dense.

LB-, no colony will be visible because media is not present.

LB amp+, only transformed cells will be able to grow as they will be harbouring ampicillin resistance gene.

LB amp-, transformed as well as non-transformed, both cells will grow. In addition, contaminant microbes may also be present.

LB naladixic acid+, transformed as well as non-transformed, both cells will grow.

LB naladixic acid-, transformed as well as non-transformed, both cell will grow. In addition, contaminants microbe may also be present.

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